Equine chorionic gonadotrophin improved vascularization of feline ovarian tissue xenografted into immunosuppressed mice.

The objective of this study was to evaluate effects of eCG on vascularization and development of feline ovarian tissue xenografted to immunosuppressed mice. Feline ovarian fragments (∼1 mm3) were transplanted under the renal capsule of 20 adult, ovariectomized, C57BL/6 SCID female mice. At 45 d after transplantation, 10 mice (controls) were euthanized and the remainder given 10 IU of eCG (and sacrificed 48 h later). Transplants were recovered immediately after death, fixed, sectioned, and stained with periodic acid-Schiff (PAS). Fragment volume (Cavallieri principle) and vascularization were assessed. Mean xenotransplant volume for control and treatment groups was 0.17 ±â€¯0.03 and 0.37 ±â€¯0.13 mm3, respectively (P = 0.0952); vascular volume density, 30.3 ±â€¯11.3 and 49.1 ±â€¯8.9% (P = 0.0281); surface density, 4.1 ±â€¯2.4 and 6.2 ±â€¯1.7 µm-1 (P = 0.2222); and vessel total surface, 0.63 ±â€¯0.24 µm2 and 2.28 ±â€¯1.05 µm2 (P = 0.0079). In conclusion, eCG significantly increased vascular volume density of xenotransplanted ovarian tissue and improved its development.

Monitoramento do ciclo estral de fêmeas equinas por meio de citologia vaginal, ultrassonografia e dosagem hormonal / Monitoring equine estrous cycle through vaginal cytology, ultrasonography and hormone dosage / Monitoreo del ciclo estral de hembras equinas por medio de citología vaginal, ultrasonido y dosis hormonal

O ciclo estral é a sincronia entre eventos comportamentais, anatômicos e endócrinos que resultam na ovulação, e pode ser dividido em fase lútea e fase folicular. A identificação das fases do ciclo estral da égua pode ser acompanhada por meio de várias biotécnicas reprodutivas como, por exemplo, citologia vaginal classificando os diversos tipos de células do epitélio vaginal, ultrassonografia para avaliação da dinâmica folicular e a dosagem hormonal no plasma sanguíneo ou das fezes do animal para caracterizar a fase do ciclo estral que o animal possivelmente se encontra. Sendo assim, o objetivo desta revisão foi discutir a fisiologia do ciclo estral de éguas, a fim de favorecer novas pesquisas relacionadas à biotécnicas reprodutivas para equinos.

The estrous cycle is the synchrony of behavioral, anatomical and endocrine events that result in ovulation. It can be divided into luteal and follicular phases. The identification of the stages in the estrous cycle of mares can be accomplished through several reproductive biotechnologies, such as, for instance, vaginal cytology classifying the different types of cells in the vaginal epithelium, follicular dynamics evaluated through ultrasound examination, and hormone dosage in blood plasma or feces from the animal to characterize the likely estrous cycle phase. Thus, the objective of this review is to discuss the physiology of the estrous cycle in mares in order to encourage new research related to reproductive biotechnologies for horses.

El ciclo estral es la sincronía entre eventos de comportamiento, anatómicos y endocrinos que resultan en la ovulación, y se puede dividir en fase lútea y fase folicular. La identificación de las etapas del ciclo estral de la yegua puede ser acompañada a través de varias biotécnicas reproductivas como, por ejemplo, citología vaginal, clasificando los diferentes tipos de células del epitelio vaginal, ultrasonido para evaluación de la dinámica folicular y la dosificación hormonal en el plasma sanguíneo o de las heces del animal para caracterizar la fase del ciclo estral que el animal posiblemente se encuentra. Así, el objetivo de esta revisión ha sido examinar la fisiología del ciclo estral de yeguas, con el fin de fomentar nuevas investigaciones relacionadas con las biotecnologías reproductivas para equinos.